A simple method for the determination of the cholesterol esterase activity.

A simple method for the determination of serum cholesterol

A Sensitive Neutralization Assay for Influenza C Viruses Based on the Acetylesterase Activity HEF Glycoprotein

Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...

Preparation of a reusable enzyme strip for determination of serum cholesterol

A reusable enzyme strip for the determination of cholesterol was prepared by co-immobilizing cholesterol esterase (CEase), cholesterol oxidase (COD) and peroxidase (POD) onto alkylamine glass beads affixed on a plastic strip by a nonreactive fixative. The co-immobilized enzymes retained 71.42% of initial activity of free enzymes with a conjugation yield of 11.0 mg g beads and showed optimum act...

A Simple and an Effective Catalyst for Determination of Cefixime in Real Samples Using of Modified Carbon Paste Electrode with Cobalt Impregnated Zeolite

Carbon paste electrode modified with cobalt impregnated zeolite was prepared by mixing of cobalt_zeolite (Co_Z) and graphite powder with different percent. The electrochemical oxidation of cefixime at such electrodes was investigated using the cyclic voltammetric and amperometric techniques. It is not electrochemically active towards cefixime oxidation in 0.1 M phosphate buffer solution (pH=7) ...

Improved method for enzymic determination of cholesterol in lipoproteins separated by electrophoresis on thin layer agarose gels.

The cholesterol of lipoproteins, separated electrophoretically on thin layer agarose films, is visualised and quantitated by incubating the gels in an enzymic reagent containing cholesterol esterase and cholesterol dehydrogenase. The individual fractions are quantitated by scanning densitometry. No sample pretreatment is necessary. All major fractions are detected readily. The accuracy of the d...